Transcardial Perfusion Histological Color Intensity and Artifact Count.xlsx

Transcardial perfusion is widely used to preserve rodent brain tissue. However, varying parameters in protocols such as fixative volume and delivery technique remain unexplored, particularly its histological quality. This dataset compares histological quality from differently perfused rat brains, indicated by mean gray values to represent the staining intensity of the nucleus and cytoplasm as well as artifact count of each groups to evaluate the effects of volume and delivery technique to the histological quality of rat brain tissue.