Single cell genomic analysis of PBMCs cells from a primary myelofibrosis patient

We performed single cell DNA sequencing by means of Tapestri platform on a mix of Peripheral Blood Mononuclear Cells (PBMCs) and CD34+ hematopoietic stem/progenitor cells (HSPCs) from a primary myelofibrosis (PMF). The main purpose of this project was to reconstruct the clonal composition of the neoplastic population by analyzing the mutational profile at the single-cell level. Overall design: CD34+ cells were isolated through immunomagnetic selection from frozen PBMCs coming from a PMF patient. PBMCs and CD34+ cells were subjected to Tapestri analysis through a custom DNA panel.