Mouse Embryonic Fibroblasts (MEFs) Adipogenic Potential: A Comprehensive Transcriptome Analysis

Exploration into the dynamic process of adipogenesis in MEF cells via transcriptome analysis Overall design: RNA isolates of MEF cells extracted at different timepoint and conditions. Timepoints included day 0,3, and 5. Conditions, for day 3 and 5, included adipocyte differentiating condition (incubation with basal growth media supplemented with 0.5 mM 3-isobutyl-1-methylxanthine , 10 µg/mL insulin, 1 µM dexamethasone, and 10 µM rosiglitazone) and non-differentiating condtion (basal growth media DMEM 10%FBS).