Pool-GWAS on D. melanogaster trident pigmentation. Trident Pool-GWAS

We used a pooled replicated genome-wide association approach (Pool-GWAS) to identify the genetic basis of variation in thoracic trident pigmentation in two Drosophila melanogaster populations. We confirmed the previously reported large effect of ebony and the associated cosmopolitan inversion In(3R)Payne. For the first time, we identified tan as another major locus contributing to variation in trident pigmentation.