Long non-coding RNA LINC00174 Acts as a miR-3713 Sponge to Deregulate Oncogenic Targets, Promoting Nasopharyngeal Carcinoma Xenograft Progression via Modulating Tumor Growth, Apoptosis, and Histopathology

Database Description: LINC00174-miR-3713 Axis in NPC
This database curates experimental data validating lncRNA LINC00174’s oncogenic role in nasopharyngeal carcinoma (NPC) via sponging miR-3713. It integrates molecular interaction, in vitro/in vivo functional assays, and histopathology data to support NPC biomarker discovery and targeted therapy research.

Core Data Modules
Molecular Interaction: LINC00174 siRNA sequence (5’-GGAUUCUAGACUUCUACUATT-3’); L-KD reduces LINC00174 by 76% and increases miR-3713 by 3.8-fold (p<0.001).
In Vitro Assays: C666-1 cell experiments (4 groups: Vector NC, siRNA NC, L-KD, L-KD+I). L-KD downregulates Ki-67 (84%), PCNA (90%), MMP-2 (88%), and upregulates E-cadherin (7.3-fold; p<0.001).
In Vivo Tumorigenesis: BALB/c-nu/nu mice xenografts. L-KD reduces tumor volume (63%) and weight (63%) on Day28; miR-3713 inhibition partially rescues these effects (p<0.001).
Histopathology: L-KD reduces nuclear pleomorphism (H&E) and increases TUNEL⁺ apoptotic cells (3.6-fold; p<0.001), reversed by miR-3713 inhibition.
Utility
Supports lncRNA-miRNA network analysis, NPC biomarker identification, and LINC00174-targeted therapy development.

Keywords: LINC00174; miR-3713; NPC; ceRNA; therapeutic target.