Multiplexed, Quantitative Workflow for Sensitive Biomarker Discovery in Plasma Yields Novel Candidates for Early Myocardial Injury.

Data from Massive, ID: MSV000078918. Experiment: Discovery_inPatient_IgY14_Supermix_depletion, PMI_Patient1, file: G20121012_PMI_P24_SMdepl_iTRAQ_basicRP_fr23.mzml. Published as part of Mol Cell Proteomics. 2015 Feb 27 . From the Abstract: {{i}} We have developed a novel plasma protein analysis platform with optimized sample preparation, chromatography and MS analysis protocols. The workflow, which utilizes chemical isobaric mass tag labeling for relative quantification of plasma proteins, achieves far greater depth of proteome detection and quantification while simultaneously having increased sample throughput than prior methods. We applied the new workflow to a time series of plasma samples from patients undergoing a therapeutic, planned myocardial infarction (PMI) for hypertrophic cardiomyopathy, a unique human model in which each person serves as their own biologic control ... {{/i}}