The diagnostic accuracy, sensitivity and specificity of 16-gene signature to discriminate AA and GBM in training set, test set and three independent cohorts (TCGA, GSE1993, GSE4422) of patient samples.

APrediction accuracy was determined by 10-fold cross-validation on malignant astrocytoma samples.Accuracy = (the number of samples predicted correctly)/(total number of samples analyzed).BSensitivity = (the number of positive samples predicted)/(the number of true positives).CSpecificity = (the number of negative samples predicted)/(the number of true negatives).*14-genes of the 16-gene signature were used for analysis.