Raw Western blots data to assess cold shock protein expression

Preclinical and clinical studies show that mild to moderate hypothermia is neuroprotective in sudden cardiac arrest, ischemic stroke, perinatal hypoxia/ischemia, traumatic brain injury and seizures. Induction of hypothermia largely involves physical cooling therapies, which induce several clinical complications, while some molecules have shown to be efficient in pharmacologically-induced hypothermia (PIH). Neurotensin (NT), a 13 amino-acid neuropeptide that regulates body temperature, interacts with various receptors to mediate its peripheral and central effects. NT induces PIH when administered intracerebrally. However, these effects are not observed if NT is administered peripherally, due to its rapid degradation and poor passage of the blood brain barrier (BBB). We conjugated NT to peptides that bind the low-density lipoprotein receptor (LDLR) to generate “vectorized” forms of NT with enhanced BBB permeability. We evaluated their effects in epileptic conditions following peripheral a..., Western blots were obtained using antibodies that detect RBM3 and CIRBP cold shock proteins and beta-actin used as an internal control, on hippocampal samples., , # Western blots

[https://doi.org/10.5061/dryad.nzs7h451x](https://doi.org/10.5061/dryad.nzs7h451x)

## Description of the data and file structure

Brains were extracted, cut in two for mRNA and protein analysis. In each hemi-section, the hippocampus was isolated and snap frozen. RT-qPCR analysis normalized with GAPDH on pooled samples showed significant increase of mRNA encoding RBM3 and CIRBP relative to PBS injected control at the 16 H and 8 H time points, respectively. In order to assess whether increased mRNA levels translate into increased protein levels in hippocampal samples, we used western blot and antibodies that detect RBM3, CIRBP and beta-actin used as an internal control. The raw data for the western blots is provided.

### Files and variables

#### File: image2CIRBP\_PBS\_VH-N412\_4H\_8H\_16H\_Ladder.tif

**Description:** Western blot to assess ladder size and the levels of CIRBP after VH-412 treatment at 4, 8, 16 hours. 

#### File: subImage1\_b-actin\_PBS\_VH-N412\_...