Investigations of the effects of subMIC antibiotics on the P. aeruginosa transcriptome

Antibiotics in low concentrations may have an array of effects. We have exposed growing P. aeruginosa cells to different classes of antibiotics in order to investigate this hypothesis. We have used DNA microarrays to investigate the effect of low concentrations of antibiotics (azithromycin, ceftazidime and ciprofloxacin) on the gene expression of P. aeruginosa aiming to elucidate general trends. Keywords: Treatment with antibiotics Exponentially growing P. aeruginosa cells were exposed to antibiotics in concentrations not affecting growth, grown for 2 hours and harvested. Untreated cell were grown likewise for reference.