Study on Low temperature Mechanism via SMRT-based RNA-seq in Chrysanthemum leaves

The mixed sample was obtained from the salt stress treatment and the control, then we obtained one cDNA library. In total, 3,717,516 subreads and 141,445 Consensus reads were constructed. Overall design: Dendranthema grandiflorum var. jinba was used in this study. The buds raised from tissue-cultured seedlings were grown on MS medium (16 h photoperiod, 25 °C/22 °C day/night temperature) for thirty days. Then thirty-day old chrysanthemum seedlings were transferred to pots filled with a 1:1 mixture of peat and perlite, and acclimated for five days at normal condition. Then the seedlings were subjected to following treatments: (CK) normal condition as control, (T) 4 °C for 24 h, followed by - 4 °C for 4 h. Each treatment collected a mixture of three biologically replicated leaves, then samples rapidly frozen with liquid nitrogen and stored at - 80 °C. There were two samples in total used for RNA-Seq.