Tissue-specific transcriptomic profiling of opium poppy (Papaver somniferum)

To facilitate genome annotation, we performed RNA sequencing of seven different opium poppy tissues (leaf, petal, stamen, capsule, stem, fine root, tap root). On the first day of anthesis material was sampled from the following seven tissue types using a subset of plants: fine roots, tap root, leaves (the two uppermost ones), stem (the 2 cm long part just underneath the capsule), capsule, petals and stamens. Five days after the onset of anthesis stem and capsule materials were collected from another subset of plants. These plants had been manually pollinated on the first day of anthesis and had shed their petals at the time of sampling.