RNAseq analysis of raf and srk2dei mutant

We are interested in ABA-mediated drought stress signaling in plants. We revealed that RAF activated subclass III SnRK2s. So, we performed transcriptomic analyses of arabidopsis raf mutant, srk2dei and aba2 mutant following a dehydration stress or ABA treatment using RNA-seq analysis to reveal a functional relationship between RAFs and subclass III SnRK2s. We used 12d-old whole seedlings of wild type (Col-0), raf mutant or srk2dei mutant plants grown on GM agar plates following a dehydration stress for 2 hours or ABA treatment as materials for RNAseq analysis. We also have three biological replicates for this analysis.