Transcriptome of LBR-null mouse kidneys

We tested overall gene expression change upon chromatin architecture disruption. RNA-seq was performed using nuclear architecture-disrupted mice carrying a loss-of-function mutation in LBR (Lbric-J/ic-J; LBR-null mice). LBR is an INM protein that tethers heterochromatin to the nuclear envelope whose level is decreased in various aging contexts. Heterozygous LBR-null mice were recovered from cryopreservation at The Jackson Laboratory (C57BL/6J-Lbric-J/J; stock number 000529). After the breeding and genotyping, three homozygous LBR-null mice (Lbric-J/ic-J) and one WT littermate were sacrificed at age of 4 weeks. When they were sacrificed, the right kidneys of mice were flash-frozen, then pulverized and aliquoted for RNA extraction. The LBR-null kidneys were lysed and homogenized in TRI Reagent (R2050, Zymo Research). Trizol RNA miniprep plus kit (R2070, Zymo Research) was used to isolate total RNA. Library preparation with poly(A) selection and 150 bp paired-end sequencing on an Illumina HiSeq were performed by Genewiz (South Plainfield, NJ).