<b>Assessment of professional soccer players' physical fitness levels based on certain gene (</b><i><b>AGT</b></i><b> rs699 &amp; </b><i><b>IL-6</b></i><b> rs1800795) polymorphisms</b>

The present investigation endeavors to elucidate the physical fitness attributes inherent to professional soccer athletes in accordance with the AGT rs699 and IL-6 rs1800795 gene polymorphisms. A cohort of twenty-two accomplished soccer players, aged between 18 to 35 years, willingly participated in the study. Subsequent to the procurement of genomic DNA from oral epithelial cells through the Invitrogen DNA isolation kit (Invitrogen, USA), in adherence to the manufacturer's guidelines, genotyping procedures were executed using real-time polymerase chain reaction (PCR). The evaluative parameters for the players' physical fitness encompassed squat jump (SJ), countermovement jump (CMJ), drop jump (DJ), Yo-Yo Intermittent Recovery test level 2 (Yo-Yo IRT 2), bench press one repetition maximum (1RM), and sprinting capacities (5m, 10m, 30m). Statistical analysis entailed the utilization of the Wilcoxon Signed Rank Test to discern differences between the pre-test and post-test measurements of the soccer participants. Upon segregating the outcomes in alignment with the AGT genotype variable, statistically significant differences were not discerned in SJ, 5m sprint time, 30m sprint time, CMJ, DJ evaluations, and body fat percentage (p &gt; 0.05). In contrast, statistically significant differences were observed in the context of Yo-Yo IRT 2, 10m sprint time, and 1RM bench press outcomes (Yo-Yo IRT 2: CC and CT p = 0.011, 0.012; 10m: p = 0.028; 1RM: CC and CT p = 0.007, 0.008 correspondingly). Upon analysis predicated upon the IL6 genotype variable, statistically significant differences were absent in SJ, 30m sprint time, CMJ, DJ appraisals, and body fat percentage (p &gt; 0.05). Nevertheless, significant differences were identified in the domains of Yo-Yo IRT 2, 5m sprint time, 10m sprint time, and 1RM bench press measurements (Yo-Yo IRT 2: CC and GG p = 0.012, 0.005; 5m: CC p = 0.045; 10m: CC and GG p = 0.010, 0.033; 1RM CC and GG p = 0.008, 0.003 respectively). In summation, the gene polymorphisms AGT rs699 (C allele) and IL-6 rs1800795 (C allele) exhibit potential associations with adaptations induced by training regimens. However, it is imperative to conduct further investigations, particularly within the confines of homogenous participant cohorts, to gain enhanced insights into the nexus between the aforementioned rs699 and rs1800795 polymorphisms and their influence upon professional soccer athletes.本研究旨在依据AGT rs699与IL-6 rs1800795基因多态性，阐明职业足球运动员所固有的体能特质。本研究共招募22名年龄介于18至35岁的优秀足球运动员作为研究对象，所有受试者均自愿参与本研究。按照制造商操作规范，使用美国Invitrogen公司的DNA提取试剂盒从口腔上皮细胞中提取基因组DNA后，采用实时聚合酶链式反应（PCR）完成基因分型实验。本研究对受试者的体能评估指标包括：蹲跳（Squat Jump, SJ）、反向跳（Countermovement Jump, CMJ）、跳深（Drop Jump, DJ）、Yo-Yo间歇性恢复测试二级（Yo-Yo Intermittent Recovery Test Level 2, Yo-Yo IRT 2）、卧推单次最大重量（1RM Bench Press, 1RM）以及短跑能力（5米、10米、30米短跑）。本研究采用威尔科克森符号秩检验（Wilcoxon Signed Rank Test）对足球运动员的前测与后测数据差异进行统计分析。按AGT基因型分组分析结果显示，受试者的蹲跳、5米短跑时长、30米短跑时长、反向跳、跳深测试以及体脂率均未呈现统计学显著性差异（p > 0.05）。与之相对，Yo-Yo间歇性恢复测试二级、10米短跑时长以及卧推1RM指标则呈现统计学显著性差异：Yo-Yo IRT 2中CC型与CT型的p值分别为0.011、0.012；10米短跑p值为0.028；卧推1RM中CC型与CT型的p值分别为0.007、0.008。按IL-6基因型分组分析结果显示，受试者的蹲跳、30米短跑时长、反向跳、跳深测试以及体脂率均未呈现统计学显著性差异（p > 0.05）。但Yo-Yo间歇性恢复测试二级、5米短跑时长、10米短跑时长以及卧推1RM指标则呈现统计学显著性差异：Yo-Yo IRT 2中CC型与GG型的p值分别为0.012、0.005；5米短跑中CC型的p值为0.045；10米短跑中CC型与GG型的p值分别为0.010、0.033；卧推1RM中CC型与GG型的p值分别为0.008、0.003。综上，AGT rs699（C等位基因）与IL-6 rs1800795（C等位基因）的基因多态性或与训练诱导的体能适应性存在潜在关联。但未来仍需开展进一步研究，尤其是采用同质化受试者队列的研究，以更深入地阐明上述rs699与rs1800795基因多态性对职业足球运动员体能的影响机制。