<i>ces-1(n703</i>gf<i>)</i>; <i>cya-1(bc416)</i> affects the division of the NSM neuroblast.

(A) The presence of NSMs, undead NSM sister cells, and non-dividing NSM neuroblasts was analyzed in L3, L4 larvae using the reporter Ptph-1his-24::gfp (bcIs66). All strains analyzed were homozygous for bcIs66. Epifluorescence images overlaid with DIC. (B) The NSM neuroblast division was analyzed in embryos using the reporter Ppie-1mCherry::phPLC1δ (ltIs44) or Ppie-1gfp::phPLC1δ (ltIs38). Epifluorescence images were taken before (‘comma’) and after the NSM neuroblast division (‘1.5-fold’). In the case of ces-1(n703gf); cya-1(bc416), the NSM neuroblasts had not divided at the time the analysis had to be terminated due to the beginning of muscle twitching (around the 2-fold stage). White arrows point to the NSM neuroblasts, blue and orange arrows point to the NSMs and NSM sister cells, respectively. All strains analyzed were homozygous for ltIs44 and bcIs66, except ces-1(n703gf), which was homozygous for ltIs38 and bcIs66. (C) Quantification of the percentage of NSM neuroblasts dividing in wild-type, ces-1(n703gf), ces-1(n703gf); cya-1(bc416), cya-1(bc416) and cdc-25.2(RNAi) embryos. cdc-25.2(RNAi) was performed by injection. n indicates the number of NSM neuroblasts analyzed.