The 16srRNA data of EcN C6 on gut microbes in the hyperuricemia rat model.

科学数据银行2022-03-21 更新2026-04-23 收录

消化道微生物组

高尿酸血症模型

数据链接：

https://www.scidb.cn/en/detail?dataSetId=94f11fea850f4aa4bcae9c344df4ff3a 数据链接链接失效反馈

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资源简介：

Feces collected from hyperuricemia rats were frozen in -80 ℃ until use. DNA was extracted using the E.Z.N.A. Stool DNA Kit (OMEGA, USA) following the instructions. 16S rDNA (V4 region) was amplified by a two-step PCR enrichment using barcodes for multiplexing. The pooled DNA was cleaned utilizing Ampure XP beads (Beckman, USA). DNA libraries were constructed using NEBNext Ultra II FS DNA Library Prep kit (NEB, USA) and were sequenced by Illumina HiSeq X Ten platform.

提供机构：

Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, China; State Key Laboratory of Virology, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences,Wuhan, China; CAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, China; ZhouWei; State Key Laboratory of Virology, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, China; Zou Linxuan

创建时间：

2022-03-10