ExtData_Figure_2_qPCR
收藏DataCite Commons2023-05-01 更新2024-08-26 收录
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https://figshare.com/articles/dataset/ExtData_Figure_2_qPCR/22710208
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<em>(B) Xrn1 knock out A549 cells were infected with WT PR8 or PR8-PA(∆X), or mock infected.</em> <em>PR8 HA RNA levels were quantified by qRT-PCR and normalized to 18S.</em> <em>(D) HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with luciferase reporters containing 99 bp insertions from </em> INSIG1,SLC7A5,STOML2,YKT6,TUBA1B or BCAP31<em> and where indicated, with PR8 PA-X. RNA was extracted and luciferase mRNA levels were quantified by qRT-PCR and normalized to 18S</em> . <em>R# --> replicate number; LG### --> experiment number</em>
提供机构：
figshare
创建时间：
2023-05-01
