Bulk RNA-seq of primary microglia with LPS stress and ApoA-I treatment

To test the functional role of ApoA-I on microglia in the context of stress, we treated primary microglia with LPS stress with or without ApoA-I protein, and used bulk RNA-seq to evaluate if ApoA-I exhibits an anti-inflammatory role. Overall design: Primary rat microglia (DIV12) were treated with LPS (100 ng/ml) for 20 hours, with or without adding ApoA-I protein (100 ug/ml) in the culture medium. We have 4 groups in total (Con, LPS, ApoA-I, LPS+ApoA-I) and 4 replicates per group.