LncRNA expression profile and their possible regulatory role in the HSOS induced by monocrotaline in rats

Aims: Long noncoding RNA (lncRNAs) contribute in regulating vital physiological processes and pathogenesis of many diseases. Monocrotaline can cause large-scale outbreaks of toxic liver disease in humans and animals as hepatic sinusoidal obstruction syndrome (HSOS). This research aimed to study the lncRNA-mRNA regulation network in MCT-induced HSOS in rats. Main methods: We established MCT-induced HSOS, and then carried out microarray to liver tissues of SD rat of an early HSOS model (MCT-12h treated and control groups) to investigate the differentially expressed lncRNAs and mRNAs in the early hepatotoxicity, followed by RT-PCR for selected lncRNAs, which were markedly changed. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genome analysis were also conducted Key findings. According to analysis, LncRNAs might play an essential role in the hepatotoxicity mechanism. Significance: This study provides a basis for further researches on the molecular mechanisms, and treatment of MCT-induced hepatotoxicity, especially in the early stage, which is pivotal to treat it successfully before irreversible liver damage occur. Microarray analysis was performed on colonic tissues from the Control and MCT (160mg/kg MCT, i.g.) groups (n=3)