The Origin Recognition Complex requires chromatin tethering by a hypervariable intrinsically disordered region that is functionally conserved from sponge to man

The first step towards eukaryotic genome duplication is loading of the replicative helicase, the Mcm2-7 complex, onto chromatin. This so-called “licensing” step is executed by the Pre-Replication Complex (Pre-RC) whose assembly on chromatin is nucleated by the DNA-binding activity of the Origin Recognition Complex (ORC). It is thought that metazoan ORC, like the yeast complex, is recruited directly to chromatin by its ATP-dependent DNA binding and encirclement activity. However, we have previously shown that this DNA binding mode is dispensable for chromatin recruitment of fly ORC, raising the question of how metazoan ORC binds chromosomes. We show here that the intrinsically disordered region (IDR) of fly Orc1 is both necessary and sufficient for the recruitment of ORC to chromosomes in vivo and demonstrate that this activity is regulated by IDR phosphorylation. In vitro studies show that the IDR alone binds DNA and this bestows the ORC holocomplex with a high-affinityATP-independentDN..., , , # The Origin Recognition Complex requires chromatin tethering by a hypervariable intrinsically disordered region that is functionally conserved from sponge to man

\##Description of data

Notes:

\**For \"EMSA\" data each includes replicate images of separate experiments and are named according to the construct (e.g., \"WT\", \"D1-D6\", or \"DBP\") and their replicate number (I.e., \"1-3\")

\**For imaging data in FIG1A-FIG1B, each has the following six components:
1\) a maximum intensity projection of the data (file name contains \"MAX\" description)
2\) the background corrected and median filtered data (file name contains \"BG_MEDIAN\" description)
3\) a sub-stack of three images derived from item 2 above (file name contains \"Substack\")
4\) three individual images in jpg format from item 3 above (file name contains \"0000\" or \"0001\" or \"0002\")

\**Imaging data in SFIG1E has the following five components
1\) a maximum intensity projection of the data (file name contains \"MAX\" description)
2\) a sub-s...