Macrophage Secretion of miR-106b-5p Causes Renin-Dependent Hypertension. Macrophage Secretion of miR-106b-5p Causes Renin-Dependent Hypertension

We generated myeloid cells lacking VDR (KODMAC) by crossing Vdrfl/fl mice with lysosome-M-promoter-driven Cre mice (Lyz2Cre) in the Ldlr-/-background (a model of diet-induced metabolic syndrome), as previously described13 and compared them to Vdrfl/flLdlr-/- littermates (control). Unbiased miRNA expression analyses using media from KODMAC or control peritoneal macrophages identified 361 differentially expressed miRNAs with p<0.05 Overall design: Supernatants were obtained from 9 independent cultures of wild-type peritoneal macrophages (wt) or of KODMAC (vitamin D receptor knockout in the macrophage lineage) (vdrko)