ITS_OTU_table.xlsx

Raw ITS OTU count table including nectar samples and controls
Raw OTU table for long-read amplicon sequencing of the fungal ITS1–ITS4 region from nectar microbiota. Columns correspond to individual libraries (nectar samples from NIL pairs 1 and 2, mock community controls, field controls, and water blanks), and rows correspond to OTUs clustered at 97% sequence identity. Values indicate the number of reads assigned to each OTU in each sample prior to filtering. Samples labelled “Mock” correspond to the ZymoBIOMICS Microbial Community Standard processed in parallel through DNA extraction, library preparation, sequencing, and downstream bioinformatic steps. Samples labelled “Water” correspond to extraction and/or PCR blanks. Field control samples (empty capillaries exposed during sampling) are also included. This table provides the unfiltered counts used to compute sequencing depth, OTU richness, dominant taxa proportions.