Allium cepa cultivar:Sherpa Exome. Allium cepa cultivar:Sherpa

Total RNA from 30 onion baseplate samples at different developmental stages (dormant, dormancy break and sprout elongation) was isolated using the PureLink RNA Mini kit (Ambion) following manufacturer instructions. Specifically, five (week 2, 8, 12, 16 and 20) and three time points (week 2, 8 and 12) were studied (in triplicate) for ethylene-treated and control bulbs, respectively. Fresh-frozen samples were ground for one minute at 30Hz with a TissueLyser II (QIAGEN) ensuring the tissue did not defrost in the process; 1 mL of lysis buffer containing 1% Beta-mercaptoethanol was added immediately after to each sample, and shaken again with the TissueLyser II for a further minute. Samples were centrifuged at room temperature to remove any debris. Clear supernatant was transferred to clean tubes and 0.5 mL of 100% ethanol added before applying samples into the spin columns. DNase treatment in column (Invitrogen PureLink DNase Set) was performed and samples were eluted with RNase-free water. Total RNA was quantified using a Nanodrop Lite Spectrophotometer (Thermo Scientific) and its quality assessed on a 2100 Bioanalyzer (Agilent Technologies, Singapore). High quality RNA (RIN >8) from three biological replicates for each sample was used for transcriptome sequencing using Illumina HiSeq 2000 platform to generate 100-nt long paired-end reads. Library preparation and sequencing was performed at Earlham Institute.