Delayed administration of TAT.ARC protein attenuates focal ischemic brain injury: Mice were subjected to 60 min MCAo and 5 µg TAT.ARC or control protein was injected 3 h after the onset of ischemia and indirect lesion volume was assessed after 3 days.

Mice were treated 3h after the onset 60 min middle cerebral artery occlusion (MCAo) with TAT.b-Gal or TAT.ARC [5µg] in the contralateral ventricle of the brain and were perfused after 72 h. Infarkt volume assessment using haematoxylin staining and the following brain coordinates to select coronal slices. Mouse brains were cut in coronal sections using a mouse brain atlas (Franklin and Paxinos, 2007) to analyze direct and indirect infarct volumes, striatal area in a3 (area 3) and neuronal densities within the striatum in a3: a1 = interaural 6.6 mm/ Bregma = 2.80; a2 = interaural 5.34 mm/ Bregma = 1.54; a3 = interaural 3.94 mm/ Bregma = 0.14; a4 = interaural 1.86 mm/ Bregma = -1.94; a5 = interaural -0.08 mm/ Bregma = -3.88.