Supplementary Datasets for "MSC1 Cells Suppress Colorectal Cancer Cell Growth via Metabolic Reprogramming, Laminin–Integrin Adhesion Signaling, Oxidative Stress Resistance, and a Tumor-Suppressive Secretome"

This archive contains the supplementary datasets supporting the analyses in the manuscript“MSC1 Cells Suppress Colorectal Cancer Cell Growth via Metabolic Reprogramming, Laminin–Integrin Adhesion Signaling, Oxidative Stress Resistance, and a Tumor-Suppressive Secretome.” by Galliou PA, et al. All files are provided in Excel format and structured to reproduce the protein-protein interaction and transcription factor (TF) target analyses downstream of TLR4 activation by LPS in WJ-MSCs. Contents of this ZIP: STRING_Input_Protein_List-for_PPI_Network_Construction.xlsx Curated list of proteins reported in the literature as downstream effectors of TLR4 upon LPS stimulation. PPI_Network_from_STRING-Downstream_of_TLR4_Activation.xlsx Protein–protein interaction network generated by STRING using the input list from File 1, including interaction scores and network metrics. MCODE_Clusters-In_PPI_Network_Downstream_of_TLR4_Activation_by_LPS.xlsx Module clusters identified within the PPI network (File 2) via the MCODE Cytoscape plugin, with cluster IDs and constituent proteins. WJ-MSC_Expressed-TF_Target_Genes-of_Enriched_TF_Targets_in_PPI_Network_Downstream_of_TLR4_Activation_By_LPS.xlsx List of transcription factor target genes (from MSigDB) of enriched transcription factor targets in the PP network (File 2) (from Metascape) that are also expressed in WJ-MSCs. Includes gene names, function, genomic location, and annotated GO terms derived from Uniprot. Usage Notes: To reproduce the PPI analysis, first load File 1 into STRING (https://string-db.org). Cluster definitions (File 3) were obtained with default MCODE parameters in Cytoscape v3.10.3. Trascriptional factor target enrichment and filtering (File 4) were performed using Metascape (https://metascape.org) against the MSigDB TF target gene sets, then cross-referenced to WJ-MSC expression data (https://www.stemformatics.org/datasets/view?id=6393). Version: v1 (2025-05-28) License: CC-BY 4.0