Assessment of potential endogenous controls, snRNA:U6, miR26a and miR191, for use in porcine studies of ALF, using miR122 as the target miRNA.

Potential endogenous controls were quantified by qRT-PCR in plasma samples from acetaminophen-treated pigs (n = 3, raw Ct). The stability of the potential endogenous controls with progression of ALF was assessed using the following software packages: Normfinder [28]; BestKeeper [29]; geNorm [30,31]; and Delta Ct method [32]. The results from these analyses were used to form the composite rank to determine the final choice of endogenous control. For a graphical representation of the stability of the endogenous controls see S1 Fig.Ct, threshold cycle; SD, standard deviation; CPs, crossing points; M-value, gene stability value; CV, coefficient of varianceAssessment of potential endogenous controls, snRNA:U6, miR26a and miR191, for use in porcine studies of ALF, using miR122 as the target miRNA.