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Single cell RNAseq analysis of organotypic brain slice microglia

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NIAID Data Ecosystem2026-03-12 收录
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE154854
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Single cell RNA sequencing analysis was performed on microglia isolated from brain slices (timepoints: 1 day, 1 week or 3 weeks in culture), 2D in vitro cultured microglia, and acutely isolated adult microglia. Differences in gene expression were examined to determine relative similarity to acutely isolated adult microglia. Microglia were isolated from cultured brain slices at 3 different timepoints, from 2D invitro mixed glial cultures, and adult mouse brain and subjected to scRNAseq analysis using the 10x Chromium system; n = 2 biological replicates per group
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2021-03-15
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