Here we describe the efficient synthesis of two oligosaccharide moieties of human glycosphingolipids, globotetraose (GalNAcβ1→3Galα1→4Galβ1→4Glc) and isoglobotetraose (GalNAcβ1→3Galα1→3Galβ1→4Glc), wi

PubMed Central30

Table 1_Targeted UDP-glucose ceramide glucosyltransferase stable overexpression induces a metabolic switch improving cell performance at high cell density.xlsx

病毒载体生产工艺优化

代谢工程

BackgroundViral vectors such as adeno-associated viruses (AAVs) and virus-like particles (VLPs) are critical tools in gene therapy, typically produced using transient gene expression (TGE). Intensific

NIAID Data Ecosystem20

Identification of Absidia orchidis steroid 11β-hydroxylation system and its application in engineering Saccharomyces cerevisiae for one-step biotransformation to produce hydrocortisone

类固醇生物合成

代谢工程

Purpose: In order to identify genes for 11β-hydroxylase CYP5311B2 and associated redox partners CPR and cytochrome b5 in filamentous fungus Absidia orchidis AS3.65 Methods: Strand-specific RNA-seq lib

NIAID Data Ecosystem50

original data for the manuscript: Reprogramming orthogonal regulatory and metabolic system for secondary metabolite production

代谢工程

红霉素研究

This dataset corresponds to the raw experimental data for the manuscript "Reprogramming orthogonal regulatory and metabolic system for efficient erythromycin production". It includes: 1. “Figure 2.xls

Mendeley Data20

Genome sequencing data of evolved strains from ALE experiments. Escherichia coli strain:BW25113

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