Identification of extensive cellular diversity and maturation of active neuronal networks in long-term cultures of human brain organoids

We analyzed gene expression in over 80,000 individual cells isolated from 31 human whole-brain organoids that has developed for 3-6 months. We find that organoids can generate a broad diversity of cells, which we show are related to known endogenous classes, including subpopulations of neurons and progenitors of the cerebral cortex Single cell Droplet sequencing of human cerebral organoid