Identification of a Broadly Fibrogenic Macrophage Subset Induced by Type 3 Inflammation

Cross-sectional single cell RNA sequencing (scRNAseq) of hepatic cells enzymatically isolated from murine models of toxin- (CCl4) or diet-induced (Gubra Amlyn Nash or GAN) liver fibrosis to identify macrophage populations associated with fibrosis. Overall design: Cells were enzymatically isolated from murine livers at the peak of fibrosis (see paper for details), FACS sorted to remove dead cells and fixed in methanol before analysis using 10x Genomics scRNAseq platform. For samples labeled MMPNeg or MMPPos, cells were additionally FACS sorted based on signal from the MMPsense dye which distinguishes active intracellular matrix metalloproteases (MMPs).