Comparative molecular assessment of implant adherent cells in smokers and non-smokers. Homo sapiens

Objective: to Identify the effect of surface texture on the modulation of gene transcription of implant adherent cells as influenced by the smoking habits of the subjects. Overall design: Eighty four cp titanium implants with surface topographies exhibiting nanoscale features (Osseospeed-OS) and microrough surface without nanoscale features (TiOblast-TiO) were placed in the alveolar bone of 11 systemically healthy subjects and 10 smoking subjects and subsequently harvested at 3 and 7 days after placement. Total RNA was isolated from cells adherent to retrieved implants. A whole genome microarray using the Affymetrix Human gene 1.1 ST Array was used to describe the gene expression profiles that were differentially regulated by the implant surfaces.