Non-alcoholic steatohepatitis disrupts diurnal liver transcriptome rhythms in mice

Circadian profiling improves target gene detection and pathway characterization in mouse models of NASH. Fatty livers show 3-hour advances in metabolic and clock gene transcript rhythms. Circadian profiling further reveals temporal changes in lipid, carbohydrate, and cholesterol metabolic transcripts. Mice were fed with choline free methionine low high fat diet (cdHFD) or normal chow during 2 weeks. Mice were then sacrificed every 6 h and liver was collected. Rnaseq was then performed and data analyzed using different methods.