File S1 - Efficicent (R)-Phenylethanol Production with Enantioselectivity-Alerted (S)-Carbonyl Reductase II and NADPH Regeneration

Supporting Information. Figure S1. The maps of co-expression plasmids. When the flexible linker was used between the mutant E228S and GDH, the SD-AS sequence was changed by (GGGGS)3. Figure S2. Asymmetric reduction of acetophenone using different coexpression systems. (A) Retention times of standard samples are as follows: (R)-PE, 13.8 min; (S)-PE, 10.8 min; acetophenone, 17.2 min. (B) E. coli/pET-G-L-MS catalyzed asymmetric reduction of acetophenone; (C) E. coli/pET-MS-L-G catalyzed asymmetric reduction of acetophenone; (D) E. coli/pET-G-SD-AS-MS catalyzed asymmetric reduction of acetophenone; (E) E. coli/pET-MS-SD-AS-G catalyzed asymmetric reduction of acetophenone; AU, arbitrary units. Table S1. Plasmids, strains and primers used (DOC)