Pervasive and Dynamic Transcription Initiation in Saccharomyces cerevisiae

All regulatory signals of gene transcription are ultimately integrated in the process of transcription initiation in the core promoters. Identification the accurate locations and activities of transcription start sites (TSSs) and core promoters are essential to gain fundamental insights into regulatory mechanisms of transcription initiation. To achieve this goal, we performed non-Amplified non-Tagging Illumina Cap Analysis of Gene Expression (nAnT-iCAGE) and identified genome-wide TSSs in the model organism Saccharomyces cerevisiae across nine growth conditions.