Zebrafish ovarian transcriptome sequencing

(1) The transcriptome sequencing of nine samples was completed, and a total of 63.66 Gb Clean Data was obtained, and the Clean Data of each sample reached 6.14 Gb and above, and the percentage of Q30 bases was 91.64 % and above.(2) The Clean Reads of each sample were compared with the designated reference genome for sequence alignment, and the alignment efficiency was 92.04% or more.(3) Functional annotation was performed for all genes, including comparison with NR [1], Swiss-Prot [2], KEGG [3], COG [4], KOG [5], GO [6] and Pfam databases, and annotation results for a total of 26929 genes were obtained.(4) Based on the comparison results, the libraries were evaluated.(5) Based on the comparison results, gene expression analysis was performed. Based on the expression of genes in different samples, differentially expressed genes were identified, and functional annotation and enrichment analysis were performed.