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Spatial transcriptomic profiling of spheroid fragments sampled by micromanipulation system

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NIAID Data Ecosystem2026-05-10 收录
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https://www.ncbi.nlm.nih.gov/sra/SRP635396
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This project investigates spatial heterogeneity in three-dimensional cultured human spheroids using a custom micromanipulation system. Viable tissue fragments were aspirated from specific regions of single spheroids and analyzed by SMART-seq-based full-length RNA sequencing. The dataset enables the comparison of gene expression between surface and inner regions, and between mono- and co-culture spheroids composed of NHDF, A549, and hMSC-derived chondrogenic cells.
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2026-01-06
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