Transcriptional Response to Dauer-Inducing Ascarosides in Late L1 in C. elegans

There are two steps in the C. elegans decision to enter the dauer-diapause lifestage. The first is a choice between L2 and L2d and the second is between L3 and dauer. We studied the transcriptional changes when well-fed worms were given a cocktail of dauer-inducing ascarosides in the late L1 phase as opposed to worms that received no such treatment. This study was done in both N2 wild-type worms, and daf-22 worms, which lack an enzyme necessary to produce endogenous ascarosides. Transcriptional response of late L1 stage worms to a cocktail of ascr#2, ascr#3, and ascr#8 in in both N2 and daf-22 backgrounds.