Summary of the mutagenesis study of the three sites of the HBGA-binding interface of Boxer virus (GI-8) predicted by sequence alignment with Norwalk virus (GI) and VA387 (GII).

1The number of “+” indicated the relative binding affinity of the wild type (WT) and mutant P particles at 10 ng/µl to HBGAs: “++++” indicates an OD450 >3.0; “+++” between 2.0 and 3.0; “++” between 1.0 and 2.0; “+” between 0.3 and 1.0; while “−” indicates an OD450 <0.3, suggesting a complete loss of binding. Type O and A saliva were from secretors containing H and A antigen, respectively.