Figure S1 - <i>Mycobacterium tuberculosis</i> Lipoprotein LprG Binds Lipoarabinomannan and Determines Its Cell Envelope Localization to Control Phagolysosomal Fusion
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Total LAM expression is unaffected by deletion of lprG. Mtb H37Ra and H37Ra ΔlprG were harvested from one-week cultures. Whole cell lysates were prepared by sonication in lysis buffer and analyzed by SDS-PAGE. Mtb lipoglycan components and control proteins were detected by Western blot with (A) anti-LprG antibody (to confirm knockout of LprG in H37Ra ΔlprG). (B) Monoclonal anti-LAM antibody (CS-35) or (C) rabbit polyclonal anti-Mtb antibody. (D) Lipoglycans were detected by carbohydrate staining. (E) Western blot with anti-DnaK was used as a loading control. Blots are from one experiment and representative of at least three independent experiments. (PDF)
创建时间：
2014-10-30
