Minibioreactor arrays to model microbiome response to alcohol and tryptophan in the context of alcohol-associated liver disease

We used an in vitro microbiota modeling system named Minibioreactor arrays (MBRAs) to study IM in continuous and controlled-flow cultures. Feces from AUD patients with or without AH were transferred to MBRAs and treated with low, normal, or high Trp concentrations and in the presence of alcohol or not. 16s IM sequencing and AhR activity were studied.