Semi quantitative RT–PCR analysis of TSC1 RNA in normal urothelial cells and mutant tumour samples, showing increased electrophoretic mobility of RT–PCR products amplified from cDNA from tumours containing 104CG () and 314AG () missense mutations

Controls are amplification reactions using RT +ve (+) and RT−ve (−) cDNA from pooled TERT-NHUC. Lower panels show HPRT RT–PCR products. Also shown are schematic representations of aberrant splicing events associated with 104C&gt;G (A) and 314A&gt;G (B) mutations.<b>Copyright information:</b>Taken from "Bladder tumour-derived somatic missense mutations cause loss of function via distinct mechanisms"Human Molecular Genetics 2008;17(13):2006-2017.Published online 7 Apr 2008PMCID:PMC2427143.© The Author 2008