QCM: real-time quantitative quality control of single-molecule localization microscopy data

This repository is related to the paper ‘QCM: real-time quantitative quality control of single-molecule localization microscopy data’ by MAILFERT et al. , Biophys Journal, 2025, doi.org/10.1016/j.bpj.2025.02.018 This dataset contains: The QCM software The raw data used to publish the article Data analyzed by QCM. Although single molecule localization microscopy (SMLM) is a powerful and informative imaging technique, it remains very time-consuming, both in terms of data acquisition, post-processing analysis and interpretation of results. All these steps require a high level of expertise on the part of the experimenters to generate reliable SMLM observations. This has led to the development of Quality Control Maps (QCM), the main features of which are: an analysis speed, which, thanks to a graphical processing unit (GPU), exceeds the performance of comparable algorithms, real-time calculation of image quality indicators, and a processing mode without parameter adjustment. Accordingly, QCM provides instant feedback at launch and during acquisition of SMLM data, enabling experimenters to assess the accuracy and robustness of SMLM data acquisition. Licence CC BY-NC-SA 4.0