Loss of RUNX1 favors mesenchymal stem cells development at the expense of hematopoiesis in hPSC-derived hematopoiesis

Our data demonstrated an unprecedented role of RUNX1 in regulating hematopoietic and mesenchymal fate decisions in human pluripotent stem cells (hPSC)-derived hematopoiesis Overall design: We designed an alternative fluorescent gene tdTomato (tdT) and venus reporter and targeted into the non-coding region of exon 3 of RUNX1 gene, which is adjacent to the proximal promoter of RUNX1 gene, using TALEN-mediated homologous recombination