MouseLight Neuron AL0007

A vast neural tracing effort by a team of Janelia scientists has upped the number of fully-traced neurons in the mouse brain by a factor of 10. Researchers can now download and browse the data in three dimensions. Inside the mouse brain, individual neurons zigzag across hemispheres, embroider branching patterns and, researchers have now discovered, can even spool out spindly fibers up to 45 centimeters long. Scientists can see and explore these wandering neural traces in 3-D in the most detailed map of mouse brain wiring yet created. The map reconstructs the shape and position of more than 300 of the 70 million neurons in the mouse brain. Previous efforts to trace the path of individual neurons had topped out in the dozens. The selectively-labeled neurons were mapped in an iterative process with two-photon microscopy. The brain is sliced in 200-micron sections and a few dozen neurons are labeled at a time and imaged. Each brain imaged yields about 50 terabytes of data, each containing mapped neurons which can be browsed via the NeuronBrowser application at https://ml-neuronbrowser.janelia.org/. Researchers interested in the complete raw data set should contact Janelia to discuss obtaining it via hardware transfer.一支由Janelia科学家组成的庞大团队进行的神经追踪研究，将小鼠大脑中完全追踪到的神经元数量提高了十倍。研究人员现在可以下载并三维浏览这些数据。在小鼠大脑内部，单个神经元在半球间蜿蜒穿梭，编织出分支图案，而研究人员最新发现，它们甚至可以延伸出长达45厘米的细长纤维。科学家们可以在迄今为止创建的最详细的小鼠大脑接线图中观察并探索这些游走的神经轨迹。该图重建了小鼠大脑中7000万个神经元中超过300万个的形状和位置。之前追踪单个神经元路径的努力最多只能追踪到数十个。通过迭代过程和双光子显微镜，对这些选择性标记的神经元进行了映射。大脑被切成200微米厚的切片，每次标记并成像几十个神经元。每张大脑图像可以产生约50太字节的数据，其中包含可通过NeuronBrowser应用程序浏览的映射神经元，应用程序的网址为https://ml-neuronbrowser.janelia.org/。对完整原始数据集感兴趣的研究人员应联系Janelia，以讨论通过硬件传输获取数据的方式。

搜集汇总

背景与挑战

背景概述

MouseLight Neuron AL0007数据集是一个详细的鼠标大脑神经元映射项目，包含了超过300个神经元的3D重建数据，这些数据是通过双光子显微镜迭代标记和成像过程获得的。数据集提供了神经元的形状和位置信息，可用于神经科学研究。