Supplementary Material for: Revolutionizing ex vivo skin imaging: 3D characterization of skin tumors with ex vivo LC-OCT. A pilot study.

Introduction
Numerous imaging techniques, such as Reflectance Confocal Microscopy (RCM), Optical Coherence Tomography (OCT), and Line-field Confocal OCT (LC-OCT), are available for skin evaluation. However, there remains a need for improved imaging methods to better understand tumor histology and enhance surgical margin detection. This study aimed to evaluate the quality of ex vivo LC-OCT images in cutaneous tumors, describe their morphological features, and correlate the findings with dermoscopy, in vivo RCM, and histology.
Methods:
A prospective study was conducted from June 2022 to July 2023, including patients aged ≥18 years with cutaneous tumors on the trunk or limbs. Clinical, dermoscopic, RCM, ex vivo LC-OCT, and histopathological images were collected from 10 tumors, including melanomas, seborrheic keratoses, basal cell carcinomas, and melanocytic nevi.
Results:
Ex vivo LC-OCT produced high-quality images equivalent to those from in vivo devices, enabling three-dimensional visualization of excised tumors. The images displayed cellular resolution with strong correlations to confocal microscopy and histology.
Conclusions:
Ex vivo LC-OCT shows promise for improving the evaluation of cutaneous tumors and may enhance dermato-oncological surgery, particularly in margin control.