Gene expression analysis of wildtype AML12 versus VWA8-null AML12 cells
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE109610
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To obtain a more complete picture of gene expression changes induced by deletion of VWA8 null cells, Affymetrix microarray analysis of gene expression was carried out using RNA isolated from wildtype and VWA8-null AML12 cells. To determine changes in gene expression in AML12 liver cells associated with deletion of VWA8, separate cultures of wildtype and knockout cells were differentiated (n=4 each). Total RNA was isolated from cells using RNAStat solution (Tel-Test Inc., Friendswood, TX) and the total RNA was purified with RNeasy and DNase I treatment (Qiagen, Valencia, CA). RNA was prepared for hybridization to Affymetrix Mouse 2.0 arrays according to manufacturer’s instructions
创建时间：
2020-11-02
