Gene expression analysis of wildtype AML12 versus VWA8-null AML12 cells

To obtain a more complete picture of gene expression changes induced by deletion of VWA8 null cells, Affymetrix microarray analysis of gene expression was carried out using RNA isolated from wildtype and VWA8-null AML12 cells. To determine changes in gene expression in AML12 liver cells associated with deletion of VWA8, separate cultures of wildtype and knockout cells were differentiated (n=4 each). Total RNA was isolated from cells using RNAStat solution (Tel-Test Inc., Friendswood, TX) and the total RNA was purified with RNeasy and DNase I treatment (Qiagen, Valencia, CA). RNA was prepared for hybridization to Affymetrix Mouse 2.0 arrays according to manufacturer’s instructions