Recognition helix (RH) amino acid sequences and B2H activities of zinc finger arrays for endogenous zebrafish gene targets.

Each OPEN zinc finger array was assigned an OZ___ designation which permits their unique identification in the web-based Zinc Finger Database (ZiFDB) program [47]. Previously published zinc finger arrays targeted to the kdr gene (isolated by B1H selection) [27] are also shown. Each nine bp target site was named as follows: “gene name or abbreviation”, ”exon number”, and “L” or “R” indicating left or right half-site. The amino acids selected in the three zinc finger recognition helices of each array are shown (residues are shown left to right in the order −1, 1, 2, 3, 4, 5, 6 numbered relative to the helix start). B2H values that fall below the cut-off of three-fold activation in the B2H system are italicized. The names of zinc finger arrays tested as ZFNs in zebrafish are shown in bold italics. Abbreviations key: DT = dopamine transporter, HIF = hif1aa, Telo = telomerase, TfR2 = transferrin receptor 2, and Grck = gridlock.