Effects of Rok on binding by DnaA in vivo1.

1 Wild type (rok+; AG174) and a rok null mutant (rok-; HM57) were grown to mid-exponential phase in LB medium. Crosslinking, immunoprecipitation with anti-DnaA antibodies, and PCR with primers to the indicated chromosomal regions were done as described (Materials and Methods). Numbers indicate the fold enrichment of the indicated chromosomal regions in the immunoprecipitate compared to a control chromosomal region ± standard deviation from six independent cultures. P values are from a one-sided Student’s t-Test. The effects of rok on DnaA binding are significant at all loci indicated, except for dnaA.Effects of Rok on binding by DnaA in vivo1.