Maternal and zygotic factors sequentially shape the tissue regionalization of chromatin landscapes in early vertebrate embryos. Maternal and zygotic factors sequentially shape the tissue regionalization of chromatin landscapes in early vertebrate embryos

ChIP-seq and RNA-seq analyses to understand the establishment of the cis-regulatory mark H3K4me1 during early embryogenesis. Overall design: We examined the spatial regionalization of H3K4me1 marking across the early Xenopus tropicalis embryo. We performed H3K4me1 ChIP-seq in ectoderm, mesoderm and endoderm samples [note that the endoderm samples were previously released in GSE118024]. Subsequently, we performed H3K4me1 ChIP-seq alpha-amanitin treated embryos (ectoderm, mesoderm and endoderm) and Otx1/Vegt/Foxh1 knockdown embryos (endoderm). We also performed single embryo RNA-seq on 15 individual embryos from 3 clutches to compare the expression variation genes with or without H3K4me1 marking.